| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Diaz, Una M (9)
|
MRLIAC |
36.47 |
37.02 |
0.55 |
| 1 |
Marotta, Antonia K (9)
|
MRHAA |
42.39 |
44.63 |
2.24 |
| 2 |
Simons, Ashley R (9)
|
MRLIAC |
37.91 |
37.07 |
-0.84 |
| 2 |
Doran, Shaelee A (9)
|
MRIA |
42.27 |
44.78 |
2.51 |
| 3 |
Bull, Wesley (9)
|
MREHY |
38.02 |
38.65 |
0.63 |
| 3 |
Kutner, Virginia T (8)
|
MRLIAC |
41.64 |
45.15 |
3.51 |
| 4 |
Hsieh, Amelia (9)
|
MRLIAC |
39.68 |
39.05 |
-0.63 |
| 5 |
Lucca, Julia (9)
|
MRHAA |
37.08 |
39.11 |
2.03 |
| 6 |
Bendersky, Hailey R (9)
|
MRWISC |
39.45 |
39.15 |
-0.30 |
| 7 |
Canales Alvizuri, Isabella N (9)
|
MRLIAC |
39.51 |
39.80 |
0.29 |
| 8 |
Nguyen, Chloe T (9)
|
MRSSC |
37.23 |
40.14 |
2.91 |
| 9 |
Wigington, Scarlett G (9)
|
MRSSC |
41.99 |
40.61 |
-1.38 |